H2AX and neoplasm: However, several B-cell lymphomas are accompanied by abnormally large, mostly non-clonal, T-cell populations as determined by CD3 staining, Thy-1 mRNA levels, and diverse TCR junctions, which may be the result of strong anti-tumor T-cell responses and/or inflammation in the tumor microenvironment (Figure S5A-D).25 Notably, CAG-A3B HCCs also manifest higher levels of the DNA damage marker γ-H2AX in comparison to adjacent normal liver tissue, which is consistent with ongoing chromosomal DNA deamination by A3B (Figure S5E-F).