In this study, we further analyzed the genotype distribution and allele frequency of RASGRF1 gene polymorphism SNP loci in patients with different degrees of myopia, and the results showed that there was no significant difference in the genotype and allele frequencies of SNPs rs939658 and rs4778879 in the RASGRF1 gene in the high myopia group and low to moderate myopia group, while the genotype frequencies and allele frequencies of rs8033417 loci of RASGRF1 gene in both groups were significantly different. This evidence concerns the gene RASGRF1 and myopia.