USP35 and neoplasm: To explore the role of USP35-FUCA1 axis in vivo, we subcutaneously inoculated the control (EV + shNC), USP35-overexpressed (USP35 + shNC), and USP35 overexpressed FUCA1-depleted (USP35 + shFUCA1-4) HT29 cells in the athymic nude mice, intraperitoneally injected water (H2O), single drug (OXA as a representative), or combined drugs (OXA + 5-FU) weekly into the mice, and routinely monitored the tumor growth.