The comparison of each macrophage subtype revealed that the abundance of cells in the R4 group gated by both F4/80hi and CD206+ was significantly decreased in TG2KO mice (Fig. 2H), suggesting that TG2 was involved in the polarization of the CD45+ CD11b+ F4/80hi CD206+ Ly6Cint M2 macrophage subtype during renal fibrosis. This evidence concerns the gene ITGAM and renal fibrosis.