The comparison of each macrophage subtype revealed that the abundance of the R4 group gated by both F4/80hi and CD206+ was significantly decreased in cystamine-treated mice after UUO (Fig. 2E), suggesting that cystamine inhibits the polarization of CD45+ CD11b+ F4/80hi CD206+ Ly6Cint M2 macrophage subtype during renal fibrosis. This evidence concerns the gene ITGAM and renal fibrosis.