To evaluate whether HIF-2 stress signaling is regulated by Acss2 in colon cancer derived cell lines, we first asked whether HIF-1α and HIF-2α were present in HCT116 and HT29 cell lines and whether their levels were affected by oxygen or glucose deprivation (Figs 1A and 2A). This evidence concerns the gene HIF1A and malignant colon neoplasm.