TJP1 and alkaline phosphatase measurement: In an experimental study conducted by Huang et al.8 no significant difference was found in the levels of ZO-1, a tight junction protein, in the acute necrotizing pancreatitis group.8 In another study, it was shown that occludin and ZO-1 expression in the intestinal tissue decreased in experimental acute severe pancreatitis.22 Sonika et al23 demonstrated that the intestinal permeability increased in patients with AP.