To determine whether the effect on the actin cytoskeleton was limited to TNBC cells and the HER2+ metastatic cancer cell line, the effects of MBQ-167 and MBQ-168 were tested in the lung adenocarcinoma cell line A549, following stimulation with EGF to induce actin-based ruffles that are under Rac/PAK regulation. Here, EGF is linked to metastatic malignant neoplasm.