We found the DLBCL cell lines were extremely dependent on these genes such as NLE1, PNPT1 and PRMT1 (Figure 13A), so we knocked out the NLE1 to validate the reliability by CRISPR mediated sgRNA in the DLBCL cell lines OCI-Ly7 and DOHH2. The gene discussed is NLE1; the disease is diffuse large B-cell lymphoma.