To explore the role of S100A11 in liver cancer progression and metastasis, we used lentivirus expressing short hairpin RNA (shRNA) targeting scramble (sh-con) or S100A11 (sh-S100A11) to stably knockdown S100A11 expression in HCCLM3 and Li-7 cells, and conducted S100A11 overexpression (OE-S100A11) cells by transient transfection in Hep3B cells. Here, S100A11 is linked to liver cancer.