The RNA expression of MARCKSL1, SPP1, BSG, CCT3, LAGE3, KPNA2, SF3B4, GTPBP4, PON1, CFHR3 and CYP2C9 in normal human hepatocyte cell line MIHA and HCC cell lines HCC-LM3(high metastatic HCC cells) and HepG2(low metastatic HCC cells) were compared by qPCR. The gene discussed is SF3B4; the disease is hepatocellular carcinoma.