Studies have shown that the number of skeletal muscle mitochondria in IR, obese and T2DM animals decreased, their volume decreased, their respiratory capacity decreased, and the expression of mitochondrial biosynthesis-related proteins (such as Tfam, COXIV, Cyt C) decreased, suggesting that: mitochondrial biogenesis disorders are closely related to metabolic disorders and skeletal muscle IR [[40], [41], [42]]. This evidence concerns the gene COX4I1 and type 2 diabetes mellitus.