Next, RNA-sequencing on baseline tumor biopsies was performed and cell deconvolution using quanTIseq revealed that the tumor lesions of patients 6 and 9 were practically void of conventional effector CD4 and CD8 T-cells and T-cell related mRNAs (Fig. 5e), whereas the other patients (patients 1, 2, and 10) did show a clear activated effector T-cell signature with relatively higher expression of immune checkpoints, including LAG-3 (Fig. 5f). The gene discussed is CD8A; the disease is neoplasm.