Additional samples from the other clinical subtypes were also assayed including 19 plasma samples from 16 patients with advanced HER2-positive (HER2+) disease, 17 plasma samples from 16 patients with advanced triple-negative breast cancer (TNBC) and 1 plasma sample from 1 patient with advanced disease but unknown HR and HER2 status. This evidence concerns the gene ERBB2 and triple-negative breast carcinoma.