We further compared the performance of STEM-PCR to benchmark technique Bisulfite-PCR (BS-PCR) sequencing19, using formalin-fixed paraffin-embedded tissue samples from 20 individuals undergoing treatment for cancer, to detect methylated sites in SEPTIN9 and SFRP2 genes (chr4:153788844)20, with sequences listed in Supplementary Table 3. The gene discussed is SEPTIN9; the disease is cancer.