Consistent with the results presented in OA chondrocytes, the cartilaginous tissues of OA rats treated with nesfatin-1 showed lower expression of phosphorylated Cofilin-2 and phosphorylated LIMK1 when compared with OA rat tissues without any treatment, indicating that nesfatin-1 also inhibited the RhoA/ROCK pathway in vivo, and thus ameliorated osteoarthritis. This evidence concerns the gene NUCB2 and osteoarthritis.