However, as shown by Dedeurwaerdere et al. all dMMR detection techniques based solely on molecular methods fail to detect up to 5–10% of dMMR tumors with demonstrated loss of MLH1/MSH2/PMS2 or MSH6 immunohistochemical staining, either for biological reasons (minimal MSI shifts) or sensitivity issues (low tumor cell percentage). This evidence concerns the gene MSH6 and neoplasm.