We then calculated CAF probability scores for individual cells isolated by serial trypsinization of patient GBM based on the absence of 5 non-CAF markers (the 4 listed above, PTPRC, EPCAM, PECAM1, and CSPG4, plus RGS5, a second pericyte marker) and their degree of expression of the 9 CAF markers listed above (Supplemental Figure 6, A–C, and Figure 1D). This evidence concerns the gene CSPG4 and glioblastoma.