For example, TMPRSS11B, MUC21 and CRNN were highly expressed in the upper right corner of the t‐SNE plot characterised by E1, while the expressions of SOX4, MDK and IFI6 were higher in the upper left corner characterised by E6 and E7 (Figure 2D), which was similar to progressive ESCC.21 This evidence concerns the gene MDK and esophageal squamous cell carcinoma.