To confirm whether G4‐CSSD590 can restore the expression of CLCA1, UGT2A3 and B3GNT6 and its role in colon cancer after the adsorption of miR‐590‐3p, we transfected Ctrl CSSD, CSSD590 and G4‐CSSD590 in HCT116 and Caco‐2 cells. This evidence concerns the gene UGT2A3 and malignant colon neoplasm.