For example, EndMT transcription factor Sox9 (q-value = 7.61E−09) and Snai1 (q-value = 1.81E−07), chemokine Ccl10 (q-value = 2.30E−06), proinflammatory MAP kinase Mapk4 (q = 1.44E−19) were hypomethylated while TJ occlusion protein Cldn5 (q-value = 1.13E−33) and cytochrome-encoding gene Cyb5r3 (q-value = 6.34E−17) were hypermethylated in gene promotor region in aging control (non-stroke) group compared to the young control group (data not shown). The gene discussed is CYB5R3; the disease is Stroke.