As for HKDC1 protein, we found that its expression level was reversely regulated in comparison to NT5DC3, in both the WTAP siRNA group and LF treatment group (P < 0.05) (Fig. 4J), further proving that LF could suppress the progression of colon cancer under a high concentration of glucose through regulating the WTAP/m6A/ NT5DC3/HKDC1 axis. This evidence concerns the gene HKDC1 and malignant colon neoplasm.