Interestingly, the knockdown of WTAP could abrogate the changing of the NT5DC3 m6A or NT5DC3 protein levels after LF treatment under a high glucose conditions, and ALKBH5 siRNA group (panel 5), YTHDF1 siRNA group (panel 6) and YTHDF3 siRNA group (panel 8) showed the similar pattern of changes as WTAP siRNA group indicating that these m6A-related genes could be regulated by LF under hyperglycemia, and WTAP showed the stongest changes (P < 0.05) (Fig. 4I, J). This evidence concerns the gene ALKBH5 and Hyperglycemia.