High ADRB3 gene expression correlated with the worst clinical outcome (overall and event-free survival) of NB patients compared to lower expression; furthermore, the positive ADRB3 gene correlation with different immune-suppressive checkpoints such as CD80, CTLA4, and B7-H3 (CD276), and the negative correlation with immune-activating checkpoints such as CD266, CD40LG and 4-1BBL confirmed the negative role played by β3-AR in the complex regulation of the immune reactivity in NB. The gene discussed is CD80; the disease is neuroblastoma.