For specific biomarkers associated with cell apoptosis, tumor metastasis and cell cycle detected by western blot, Fig. 3H showed that up-regulated apoptosis regulators Bcl-2 level and down-regulated Bax level, increasing Vimentin expression and decreasing E-cadherin protein level and the elevating CyclinD1 level were observed in HCG18 overexpression group compared to NC group. This evidence concerns the gene CCND1 and neoplasm.