Included in proof of concept; improvement of IRI severity, elimination of primary nonfunction, reduction of inflammatory cytokines such as TNF-α, improvement of liver function, reduction of bile salt toxicity, and increase in survival in a stringent pig DCD model of LT10 Improvement of kidney reperfusion injury and acute kidney failure, decrease of complement activation and ROS formation in mice in situ reperfusion model41. This evidence concerns the gene TNF and acute kidney injury.