ASZ1 and hepatocellular carcinoma: Huh7.5 human hepatoma cells were transfected with in vitro transcribed RNA from a recombinant version of an HEV genome derived from the KernowC1/p6 strain (Shukla et al., 2011) in which ORF2 and ORF3 are replaced by a secreted version of Gluc (Shukla et al., 2011), termed Kc1/p6 Gluc (Figure 1A).