Following our previously established successful transcomplementation approach for studying HEV ORF3’s viroporin function (Ding et al., 2017) and cis-regulatory elements responsible for regulating transcription of the subgenomic RNA (Ding et al., 2018b), we lentivirally expressed a wt, pol (-), or C483A version of ORF1, or the pPCP alone in HepG2C3A human hepatoma cells (Figure 2A). The gene discussed is ASZ1; the disease is hepatocellular carcinoma.