NOS2 and infection: In addition to the 72 hpi timepoint used for RNA-seq, we found that infection with so/sp Mtb elicited significantly higher levels of expression of Il1b, Nos2, Il6, and Tnf at 6 and 24 hpi by qPCR compared with 5μmF preparations, with the greatest difference seen early in infection (Figure 1E–F).