Furthermore, 8 dogs in stage D had clinical signs, for example, cough (6/8), exercise intolerance (4/8), dyspnea (2/8), or tachypnea/labored respiration with pulmonary edema (2/8), and were treated with furosemide (8/8), pimobendan (8/8), spironolactone (7/8), Sildenafil (3/8), or ACE inhibitors (1/8). The gene discussed is ACE; the disease is pulmonary edema.