Furthermore, we analyzed RNA sequencing (RNA-seq) data from HepG2.2.15 cells silencing or overexpressing APOH. We further constructed an ApoH gene-knockout mouse model and a persistent HBV replication mouse model to investigate the associations between APOH levels and hepatocyte steatosis, gut microbiota dysbiosis, and HBV infection. This evidence concerns the gene APOH and steatosis.