To characterize antioxidant gene expression in A549 cells after infection with the H5N1 subtype influenza virus 24 h, relative quantitative levels of NRF2, HO-1, NADPH, GCLC, GCLM, GPX2, and SLC7A11 gene expression levels were detected using qRT-PCR. The gene discussed is HMOX1; the disease is infection.