To verify if c-Jun binds to the VIM gene promoter, we performed an electrophoretic mobility shift assay (EMSA) using nuclear extracts from normal human astrocytes (NHA) as a control, GII glioma-derived cell cultures (WG12) and two human established cell lines derived from GIV (LN18, LN229). The gene discussed is JUN; the disease is central nervous system cancer.