When we examined DNA methylation levels at the promoters of c-Jun and its targets in GII/GIII and GIV gliomas, we found differences in DNA methylation in putative c-Jun target genes S100A10, S100A2, IFRD1, RIN1, RAB36, UPP1, SLFN12 and VIM. DNA methylation patterns varied primarily in the flanking regions of the c-Jun binding sites. This evidence concerns the gene IFRD1 and central nervous system cancer.