Among them, studies have proved that CCNB1, RACGAP1 and NUSAP1 promote the progression of multiple cancers (Aljohani et al. 2022; Gu et al. 2022; Guo et al. 2020), and NCAPG and CCNB2 have been found to be associated with a poor prognosis of ES (Kakar et al. 2022; Zhao et al. 2022), while ASPM, CENPF, CKS2, and DTL have not been studied in ES to the best of our knowledge. The gene discussed is CCNB2; the disease is Ewing sarcoma.