To our interest, several genes on our list have been reported to be involved in the progression of ES, such as NKX2-2, HMCN1, HOXD10, and PRKCB. NRX2-2 participates in the formation of the core regulatory circuitry, activates EWS–FLI1 transcription, and significantly promotes the proliferation of ES cells both in vitro and in vivo (Sarver et al. 2015; Shi et al. 2020; Surdez et al. 2012; Svoboda et al. 2014). Here, FLI1 is linked to Ewing sarcoma.