The upregulation of FOXO1 and/or FOXO3 in cells undergoing apoptosis after blockade of the PI3K pathway suggested the involvement of FOXO1/3 in the induction of PUMA and BIM, as previously reported in cell lines derived from hepatocellular carcinoma and colorectal cancers [30, 31]. This evidence concerns the gene FOXO1 and hepatocellular carcinoma.