Notably, our mRNA sequencing and KEGG (Kyoto Encyclopedia of Genes and Genomes) analyses showed a negative correlation between GRAF1 and the MAPK pathway (Supplementary Fig. S8, A and Supplementary Table S4), and GESA (Gene Set Enrichment Analysis) analyses showed that CEMIP was positively associated with MAPK pathway (Supplementary Fig. S8, B), suggesting that CEMIP promoted the migration and invasion of CRC cells though GRAF1/CDC42/PAK1/MAPK axis. Here, CEMIP is linked to colorectal carcinoma.