DUOX2 c.1588A > T, SLC22A5 c.1400C > G, SLC25A13 c.852_855del, DUOX2 c.3329G > A, DUOXA2 c.738C > G, ACADSB c.1165A > G, and ACADSB c.655G > A were selected, corresponding to the biochemical indicators thyroid-stimulating hormone (TSH) (Fig. 2a), free carnitine (C0), citrulline (CIT) (Fig. 2b), and methylcrotonyl carnitine (C5) (Fig. 2C), excluding the deafness-related GJB2 and SLC26A4 and the non-C-NBS gene ATP7B. Only single-variation samples were selected for biochemical indicator analysis. This evidence concerns the gene DUOX2 and deafness.