DNAzymes showed an inhibitory effect on the expression of LMP1 and its downstream pathways, including the AP1, NF-κB and JAK/STAT pathways, which results in DNA damage and cell cycle arrest, promotion of apoptosis, and enhancement of radiosensitivity in both in vitro and in vivo NPC models. The gene discussed is NFKB1; the disease is nasopharyngeal carcinoma.