To further validate the results of bioinformatic analysis, we collected the bone marrow samples from 7 healthy donors (HD) and 13 MM patients and performed qRT-PCR to measure the mRNA levels of the relevant FRGs (AKR1C3, CP, CDKN2A, MIF, PRDX6 and TF), mostly consistent with the results which we have described before. This evidence concerns the gene MIF and Miyoshi myopathy.