We stratified all HCC patients into two groups according to AFP level (≤400 ng/mL and >400ng/mL), tumor number (single and multiple) and largest tumor size (≤5cm and >5cm), though no statistically significant difference in Metabolites-Score were found between any two groups (P>0.05, Figures 5A–C). This evidence concerns the gene AFP and hepatocellular carcinoma.