Since O-glycoproteins carrying Tn, STn, T, Sialyl T (Siaα2-3Galβ1-GalNAc-Ser/Thr; ST), and di-Sialyl T (Siaα2-3Galβ1-3[Siaα2-6] GalNAc-Ser/Thr; di-ST) antigens, which have received attention as cancer-related antigens were targeted as diagnostic markers for advanced CRC, Maclura pomifera lectin (MPL), jacalin, and Sambucus nigra lectin (SNL), which have affinity for these antigens, were used for consecutive lectin affinity purification. This evidence concerns the gene MPL and colorectal carcinoma.