By screening the whole-exome sequencing (WES) data from a discovery cohort of 87 European patients with sALS and two replication cohorts comprising 2,853 individuals with sALS, the researchers discovered 24 rare protein-coding variants in TP73. To further validate the pathogenic role of TP73 variants in ALS, they then performed functional experiments including C2C12 myoblast differentiation assays in vitro and spinal motor neuron (SMN) axonal branching assay in vivo. Here, TP73 is linked to amyotrophic lateral sclerosis.