By combining microarray and RT-qPCR results, we reported the dysregulation of hsa-miR-34a-3p and hsa-miR-1306-3p in ALS patients with SOD1/C9orf72 mutations, and hsa-miR-199a-3p and hsa-miR-30b-5p in patients with SALS, suggesting the different mechanisms underlying the gene-mutant ALS and SALS. This evidence concerns the gene C9orf72 and amyotrophic lateral sclerosis.