According to the criteria of candidate miRNAs selection mentioned in the Section ‘‘Materials and methods,’’ a total of 14 miRNAs, including two SOD1-ALS exclusively (hsa-miR-3928-3p and hsa-miR-340-5p), three C9orf72-ALS exclusively (hsa-miR-199a-3p, hsa-miR-30b-5p, and hsa-miR-485-5p), and nine overlapped both in SOD1 and C9orf72-ALS (hsa-miR-1915-3p, hsa-miR-181d-5p, hsa-miR-4729, hsa-miR-4455, hsa-miR-34a-3p, hsa-miR-1306-3p, hsa-miR-6824-5p, hsa-miR-501-3p, and hsa-miR-103a-2-5p), were found to be highly expressed in the brain tissue8 and were selected to be validated by RT-qPCR. Here, C9orf72 is linked to amyotrophic lateral sclerosis.