To ensure the stability and reliability of the above results, an external dataset (GSE34608) was used to test the expression of candidate key genes, revealing that SPATS2L, RTP4, RSAD2, OAS3, IFI44L, IFI44, CXCL10, and CD38 were upregulated in TB patients compared to healthy controls. The gene discussed is RTP4; the disease is tuberculosis.