Another gene that was upregulated in the DKK3 transfected PC3 cell line compared to the empty vector and confirmed to be upregulated in BPH samples compared to PCa samples is HIST1H2BB (Histone Cluster 1 H2B Family Member B), which is a protein-coding gene, recently described as a tumor suppressor gene (21). Here, DKK3 is linked to benign prostatic hyperplasia.