To better understand the mechanisms underlying TIGIT upregulation and DNAM-1 downregulation on CD56bright ihNK cells, we co-incubated pbNK cells with hepatoma cells or hepatocyte organoids in direct cell-to-cell contact or in a transwell setup, and compared the differential expression levels of TIGIT and DNAM-1 on CD56bright pbNK cells. This evidence concerns the gene CD226 and hepatocellular carcinoma.