CCL2 and alkaline phosphatase measurement: The chemokine analysis, revealed that CCL5, XCL1, CXCL2, CCL2, and CXCL8 were significantly upregulated in the A and AP, groups whereas the cytokine analysis showed that EBI3, GDF5, GPI, IL21, TNFSF10, and IL1A, were significantly upregulated in the A and AP groups (Figure 4I).