For example, Figures 5(a) and 5(b) are the ultrasound images of a 56-year-old female breast cancer patient before and after NAC, ER positive (0 points), the disappearance of hyperechoic halo after NAC (38 points), Alder grade 0 (100 points) points), the diameter change reached PR (28 points), and the morphological changes were more regular (30 points). This evidence concerns the gene ESR1 and breast carcinoma.