As an early model to assess obesity associated HFpEF development, mice on WD had no significant increase in nitrosative stress or cardiac fibrosis, two players in the pathogenesis of HFpEF, indicated by no changes in nos2 and col1a2 (Figures 1G, H) expression levels. This evidence concerns the gene COL1A2 and obesity due to melanocortin 4 receptor deficiency.