RHO and osteosarcoma: Human osteosarcoma(Saos-2) cells were incubated for 1 h with rhodamine-tagged tMeCP2-Rho, ZF-tMeCP2-Rho, Tat-tMeCP2-Rho, or ZF-tMeCP2(T158M)-Rho atconcentrations between 0.5 μM and 2 μM (Figure 2A).