PSMB5 and tuberculosis: We have 3 cases of mNGS results are not consistent with the result to cultivate, not included in this study, of which 1 patient mNGS results show that for the composite group of mycobacterium tuberculosis, culture result as Escherichia coli, but the patients with X - pert diagnosis of mycobacterium tuberculosis, so we consider culture specimen pollution, subsequent use anti-TB drug therapy, the prognosis is good.