In this study, we observed that the heterozygous mutations of PAX6 (c.220A>T/p.Ser74Cys) and WFS1 (c.2070_2079del/p.Cys690TrpfsTer17) were associated with more severe congenital cataracts than the monogenic heterozygous mutation of PAX6 (c.220A>T/p.Ser74Cys) in a Chinese family. The gene discussed is WFS1; the disease is early-onset non-syndromic cataract.