In males, no significant differences were found in body weight, insulin sensitivity, or glucose tolerance between castrated Cre+ and Cre− mice after DT injection (Fig. 2d–f); however, both groups developed hepatic steatosis (Fig. 2g, h), indicating that, in contrast to females, fatty liver in males is triggered by the loss of testicular function and revealing an additional sexually dimorphic effect of gonadotrope ablation on metabolism. Here, INS is linked to fatty liver disease.